If you want the second folder to stay updated with the potential changes that appear in the original folder after working with it, then yes, you would need to copy it again.
[ASK]Website loaded but not shown on BAS Browser
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Maybe our problem is related with flash or something, see this thread http://community.bablosoft.com/topic/2321/twitter-users-to-tag-not-coming-up-in-browser/6

