it can be done using txt file or database. Just create a resource as db or lines from file . Now use PARSE LINE option in TOOLS .Parse in same order as data in txt file or db . Ex user,pass,email,cookie etc. Untick Reuse Resource will make load new line every time you parse.
If you want the second folder to stay updated with the potential changes that appear in the original folder after working with it, then yes, you would need to copy it again.