If you want the second folder to stay updated with the potential changes that appear in the original folder after working with it, then yes, you would need to copy it again.
How to change ip after every load of page in browser
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@mrezair You need to use proxy action every time before page load.
Here is tutorial and video, which explains it in more details
http://wiki.bablosoft.com/doku.php?id=multithreading
http://wiki.bablosoft.com/doku.php?id=how_to_set_proxy
https://www.youtube.com/watch?v=FCfcgDX8ezk