If you want the second folder to stay updated with the potential changes that appear in the original folder after working with it, then yes, you would need to copy it again.
@BAS-Viet-Nam Okay, thank you for the hint. Worked on many sites with these 2 functions without any problems. So I will try it with "Wait while element exists" on this specific site.
Hopefully I don't get in an endless waiting-loop ;)